Prohibitin 2 antibody (Middle Region)

Catalog No. ABIN3032317

This Rabbit Polyclonal antibody specifically detects Prohibitin 2 in WB, IHC (p) and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Prohibitin 2 antibody (Middle Region) (ABIN3032317)

Target: Prohibitin 2 (PHB2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Prohibitin 2 Antibody

Binding Specificity: Middle Region

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human REA/PHB2 (RPNAQELPSMYQRLGLDY) was used as the immunogen for this Prohibitin 2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Prohibitin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Prohibitin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Prohibitin 2

Target: Prohibitin 2 (PHB2)

Alternative Name: Prohibitin 2

Background: Prohibitin 2, also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), PHB2 suppressed activation of a reporter gene in a dose-dependent manner. It had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of PHB2 were required for its repressor activity.

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling